Pglo Restriction Map. Plasmid Map Creation and Annotation Workflow DNASTAR In this experiment, pGlo (or pGFPuv) will be used as a plasmid. Customize plasmid maps with flexible annotation and visualization controls; Automatically generate a rich graphical history of every edit and procedure
Plasmids 101 Restriction Cloning from blog.addgene.org
The restriction mapping of the pGLO plasmid will be divided into three laboratory periods The pGLO plasmid is designed to enable (1) replication of the plasmid in bacteria, (2) selection of transformants using ampicillin resistance, and (3) regulation of GFP expression
Plasmids 101 Restriction Cloning
Chang Liu's lab contains the insert Green Fluorescent Protein with 1 UAG codon and is published in Integr Biol (Camb) Chang Liu's lab contains the insert Green Fluorescent Protein with 1 UAG codon and is published in Integr Biol (Camb) araC P BAD GFP ampR ampR promoter ori NdeI (4,927) ClaI (2) EcoRV (386)
Restriction map of the mobilizable plasmids pGM1190 and pGM1192 (a. associated restriction enzymes and the number of the nucleotide just before the cut site The pGLO plasmid is designed to enable (1) replication of the plasmid in bacteria, (2) selection of transformants using ampicillin resistance, and (3) regulation of GFP expression
SOLVED Title pGlo Plasmid Gene Map Consider the following example of. Before you start working with any plasmid, it is advisable to linearize it by cutting with a restriction enzyme and sequencing it via PCR to check that the advertised size and sequence are correct. Use a restriction map to predict how many fragments will be produced in a given restriction digest.